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. 2020 Jun 15;40(6):BSR20200740. doi: 10.1042/BSR20200740

Figure 4. HULC functions as a sponge for miR-204-5p.

Figure 4

(A) MiRcode software was employed to predict the target miRNAs of HULC. (B) After HUVECs were co-transfected with HULC WT or HULC MUT and miR-204-5p or miR-NC, the luciferase activities were analyzed using dual-luciferase reporter assay. (C,D) RIP assay was carried out in HUVECs, and the levels of HULC and miR-204-5p were measured by qRT-PCR. (E) The expression of miR-204-5p in HUVECs transfected with si-HULC or si-NC was examined by qRT-PCR. (F) MiR-204-5p expression in serum of sepsis patients compared with healthy controls was measured by qRT-PCR. (G) MiR-204-5p expression was determined by qRT-PCR in HUVECs treated with LPS. (H) The correlation between HULC expression and miR-204-5p expression was assessed by Pearson correlation coefficient. *P<0.05.