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. 2020 Jun 15;40(6):BSR20200740. doi: 10.1042/BSR20200740

Figure 5. Suppression of HULC increased cell viability and restrained apoptosis, inflammatory response and oxidative stress by targeting miR-204-5p in LPS-induced HUVECs.

Figure 5

HUVECs were treated with LPS for 48 h, and cells in control group were not given any treatment, and then LPS-treated HUVECs were transfected with si-NC, si-HULC, si-HULC + anti-miR-NC or si-HULC + anti-miR-204-5p. (A) MiR-204-5p expression was detected by qRT-PCR. (B,C) The cell viability and apoptosis were evaluated by using MTT and Flow cytometry assays, respectively. (D,E) Western blot assay was performed to examine the protein levels of Bcl-2, Bax, cleaved-caspase3, TNFα, IL-6, IL-8 and IL-1β. (F–H) ROS, SOD and MDA productions were determined by using DCFH-DA method and a commercial kit. *P<0.05.