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. 2020 Jun 15;40(6):BSR20200740. doi: 10.1042/BSR20200740

Figure 6. The 3′ UTR of TRPM7 was directly targeted by miR-204-5p.

Figure 6

(A) StarBase v2.0 was predicted that there were binding sites between miR-204-5p and TRPM7 3′ UTR. (B) Dual-luciferase reporter assay was performed to confirm the correlation between miR-204-5p and TRPM7, and the luciferase activity of transfected HUVECs was detected. (C,D) The mRNA and protein levels of TRPM7 in HUVECs transfected with anti-miR-NC or anti-miR-204-5p were assessed by qRT-PCR and western blot, respectively. (E,F) TRPM7 mRNA and protein levels in serum of sepsis patients and healthy controls were examined by qRT-PCR and western blot, respectively. (G,H) After HUVECs were treated with LPS or without any treatment, TRPM7 mRNA and protein levels were determined by qRT-PCR and western blot, respectively. (I) The correlation between the expressions of miR-204-5p and TRPM7 was analyzed using Pearson correlation coefficient. *P<0.05.