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. 2020 Jun 15;40(6):BSR20200740. doi: 10.1042/BSR20200740

Figure 7. MiR-204-5p promoted the development of LPS-treated HUVECs by down-regulating TRPM7 expression.

Figure 7

After LPS-treated HUVECs were transfected with miR-NC, miR-204-5p, miR-204-5p + vector or miR-204-5p + TRPM7, and cells in control group were not given any treatment. (A,B) TRPM7 mRNA and protein levels were measured by qRT-PCR and western blot, respectively. (C,D) The viability and apoptosis of transfected HUVECs were assessed by MTT and Flow cytometry assays, respectively. (E,F) The protein levels of Bcl-2, Bax, cleaved-caspase3, TNFα, IL-6, IL-8 and IL-1β were detected using western blot assay. (G–I) The productions of ROS, SOD and MDA were assessed by DCFH-DA method and a commercial kit. *P<0.05.