Figure 3:

3a. MCF-7 Percent Cell Death. Cell death did not differ between MCF-7 breast cancer cells pretreated with WR-1065 and their untreated controls at 10 Gy and 20 Gy. Breast cancer cells pretreated with radio-protective doses of WR-1065 attained the same level of cell death as untreated breast cancer cells. (10 Gy: 52.0% vs. 42.6%, p>0.05; 20 Gy: 68.8% vs. 56.4%, p>0.05) 3b. MCF-7 Microscopic Images. There was decreased breast cancer cell density and notable changes in cell morphology at 10 Gy compared to 0 Gy, with an even further reduction in cells at 20 Gy compared to 10 Gy. There was no notable difference in cell density between breast cancer cells pre-treated with WR-1065 compared to the controls at 0 Gy, 10 Gy, and 20 Gy, respectively.