Fig. 5.

Sevoflurane reduces GLUT3 protein expression in primary hippocampal neurons. a Primary hippocampal neurons were transfected with LV5-GLUT3. The transfection efficiency was examined by GFP expression (green) 36 h post infection, which was analysed by phase contrast (left panel) and fluorescence microscopy (right panel). b WB analysis of GLUT3 protein expression in primary hippocampal neurons 24 h after the last exposure to sevoflurane. Neurons were normally cultured (the Con group), only transfected with LV5-GLUT3 (the LV5-GLUT3 group), transfected with LV5-GLUT3 and then exposed to sevoflurane (the LV5-GLUT3 + Sev group), and only exposed to sevoflurane (the Sev group). c Immunofluorescence of GLUT3 expression in the somata and dendrites of primary hippocampal neurons 24 h after the last exposure to sevoflurane. The four groups: the LV5 group (neurons transfected with LV5 empty vectors), the LV5 + Sev group (neurons transfected with LV5 empty vectors and then exposed to sevoflurane), the LV5-GLUT3 and the LV5-GLUT3 + Sev group. d Histograms showing the results of GLUT3 blots in neurons among the four groups. n = 5 for each group. *P < 0.05 versus the Con group. ^#P < 0.05 versus the LV5-GLUT3 group. e Histograms showing the intensity of GLUT3 in the four treatment groups. n = 5 for each group. *P < 0.05 versus the LV5 group. ^#P < 0.05 versus the LV5-GLUT3 group. The data are presented as the mean ± s.e.m