Fig. 7.

Sevoflurane induces neural apoptosis in the hippocampus and temporal lobe. a Representative images showing apoptosis in the CA1 region of the hippocampus and temporal lobe of young mice 24 h after the third exposure to sevoflurane. Apoptosis was indicated by TUNEL immunofluorescence (green), and nuclei were stained with DAPI (blue). b Representative quantification of TUNEL-positive cells in the CA1 region of the hippocampus and temporal lobe. n = 4 for each group. In the CA1 region of the hippocampus, *P < 0.05 versus the Con group. In the temporal lobe, ^#P < 0.05 versus the Con group. The data are presented as the mean ± s.e.m