Fig. 8.

Sevoflurane induces neural apoptosis in the hippocampus and temporal lobe. a Representative immunoblots showing the protein expression of Bax, Bcl-2, cleaved caspase-3 and cleaved PARP in the hippocampus and temporal lobe of young mice 24 h after the last exposure to sevoflurane. n = 4 for each group. b, c, d, e Histograms showing the results of Bax, Bcl-2, cleaved caspase-3, and cleaved PARP blots in the hippocampus and temporal lobe. *P < 0.05 versus the Con group. In the temporal lobe, ^#P < 0.05 versus the Con group. f Representative images showing the flow cytometry analysis of the apoptosis ratio in PC12 cells 24 h after the last exposure to sevoflurane. Early apoptotic populations (PE⁺7-AAD⁻cells) are in the lower-right quadrant; late apoptotic cells (PE⁺7-AAD⁺) are in the upper-right quadrant in each dot plot. g Representative quantification of the flow cytometry analysis of the apoptosis ratio of PC12 cells 24 h after the last exposure to sevoflurane. h Representative CCK-8 assay showing cell viability in primary hippocampal neurons 24 h after the last exposure to sevoflurane. n = 5 for each group. *P < 0.05 versus the Con group. ^#P < 0.05 versus the LV5-GLUT3 group. The data are presented as the mean ± s.e.m