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. 2020 May 19;47(6):4331–4344. doi: 10.1007/s11033-020-05525-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — RT-qPCR analysis of PpARFs at different stages of fruit development in 'Okubo' (melting) and 'Jingyu' (stony hard) cultivars. a The PpARFs that were more highly expressed in 'Okubo' than in 'Jingyu' at maturity stages (S4-1 to S4-3). b The PpARFs that were more highly expressed in 'Jingyu' than in 'Okubo' at maturity stages (S4-1 to S4-3). c The PpARFs that displayed no significant difference between 'Okubo' and 'Jingyu'. The relative mRNA levels in 'Okubo' S1fruit were used as a reference (relative mRNA level set as 1). All genes were analyzed using an algorithm based on variances calculated by the cross-gene error model (± SD) in GraphPad Prism 6.0. Asterisks indicate statistically significant differences as determined by a Student’s t-test (*P < 0.05, **P < 0.01)