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. 2020 Jun 15;10:9654. doi: 10.1038/s41598-020-66561-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mir34a knock out results in an inflammatory phenotype. (A) Average of the transdifferentiation rate of acinar cell explants from pancreata of Kras^G12D; Mir34a^Δ/Δ and Kras^G12D mice into acini, duct-like and duct structures (N ≥ 3 per group). Unpaired Student’s t-test with combined SD. (B) RNA expression of members of the NFKB signalling pathway: Tnfa, Nfkb, Il6, and Nfkbia, in acinar cell explants directly after isolation (day 0) from Kras^G12D; Mir34a^Δ/Δ mice compared to Kras^G12D controls. Welch’s t-test (N = 4 per group). (C) Immunohistochemistry staining for CD45 in tissue from Kras^G12D; Mir34a^Δ/Δ mice compared to Kras^G12D controls. Scale bar 50 µm. (D) Quantification of CD45 positive cells per high power field in areas of normal tissue from Kras^G12D; Mir34a^Δ/Δ mice compared to Kras^G12D controls (N ≥ 3 per group). (E) Immunohistochemistry staining for NFKB in tissue from Kras^G12D; Mir34a^Δ/Δ mice compared to Kras^G12D controls. Scale bar 50 µm. (F) Quantification of NFKB positive nuclei from acinar cells in areas of normal tissue per high power field from Kras^G12D; Mir34a^Δ/Δ mice compared to Kras^G12D controls (N ≥ 3 per group). (G) Immunohistochemistry staining for P-STAT3 in tissue from Kras^G12D; Mir34a^Δ/Δ mice compared to Kras^G12D controls. Scale bar 50 µm. (H) Quantification of P-STAT3 positive nuclei from acinar cells in areas of normal tissue per high power field from Kras^G12D; Mir34a^Δ/Δ mice compared to Kras^G12D controls (N ≥ 3 per group).