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. 2020 Jun 15;11(6):463. doi: 10.1038/s41419-020-2625-7

Fig. 2. Absence of ANXA1 and RIG-I abrogates the reduction of cell viability in H1N1 infection of A549 cells.

Fig. 2

Cells were infected with 1 MOI H1N1 PR8 for the stated times. a Cell viability was determined using Cell Titer assay. Absorbance was read at 490 nm 2 h after addition of cell Titer substrate. b Lysates were collected at stated time points and assayed for the indicated proteins. Blots are representative of three independent experiments. c Cleaved caspase 3 (both 15 and 17 kDa) was quantified and normalized to actin. Data is represented as mean ± SEM of n = 3 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 vs. controls, #P < 0.05; ##P < 0.01; ###P < 0.001 vs. A549 parental cells using two-way ANOVA and Bonferonni post-tests.