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. 2020 Jun 15;11(6):463. doi: 10.1038/s41419-020-2625-7

Fig. 6. Re-expression of ANXA1 in A549∆ANXA1 restored IRF3 activation when RIG-I is activated.

Fig. 6

Western blot of ANXA1 in A549 and A549∆ANXA1 cells transfected with pEV (pCMV10-3xFLAG) or pANXA1 (pCMV10-3xFLAG-ANXA1) for 24 h before transfection with 1 μg/ml of 5′ppp-RNA. Proteins that were probed were a ANXA1 and b p-IRF3 and T-IRF3, respectively. Actin was used as protein loading control. Densitometry analysis of p-IRF3 and total IRF3 levels normalized to protein loading control. Data is represented as mean ± SEM of n = 3 independent experiments. c Immunoprecipitation of A549 treated with Lyovec and 5′PPP after 20 h using anti-ANXA1 antibody for pulldown and probed with RIG-I, TBK1, and ANXA1 levels.