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. 2020 Jun 15;11(6):457. doi: 10.1038/s41419-020-2651-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a qRT-PCR analysis of relative mRNA expression of Sox2 in MCF-7 and ZR75-1 cells stably infected with lentivirus carrying EV or Snail. b qRT-PCR analysis of relative mRNA expression of Sox2 in MCF-7 and ZR75-1 cells transfected with control siRNA or Snail siRNA. c Representative FACS of MCF-7 cells infected with the indicated plasmids CD44-FITC and CD24-PE. Statistical analysis of CD24⁻/CD44⁺ rates is shown in the right panel. Results shown are mean ± SD of three independent experiments. d Mammosphere assay of MCF-7 cells infected with indicated plasmids. Graphs show the relative number of mammospheres. Results shown are mean ± SD of three independent experiments. Scale bar, 20 μm. e qRT-PCR analysis of relative mRNA expression of endothelium markers VEGFR2, CD31, and CD105 in MCF-7 and ZR75-1 cells stably infected with the indicated plasmids. f FACS analysis of MCF-7 cells infected with indicated plasmids using CD31-FITC antibodies. Statistical analysis of CD31⁺ rates is shown in the right panel. Results shown are mean ± SD of three independent experiments. g Representative confocal images of MCF-7 cells infected with the indicated plasmids labelled with endothelium markers CD31 and VWF. The nuclei were stained with DAPI (blue). Scale bar, 20 μm. Graphs show the percentage of endothelium marker-positive cells. Results shown are mean ± SD of three independent experiments. h Lineage tracing of MCF-7 cells stably infected with EV or Snail using CD31pro-GFP, CD105pro-GFP, or CD144pro-GFP. Scale bar, 10 μm. Graphs show the percentage of GFP-positive cells. Results shown are mean ± SD of three independent experiments. i Tube-forming assays and DiI-AcLDL uptake assays with Snail-copGFP-expressing or copGFP-expressing MCF-7 cells. Statistical analysis of tube length is shown in the right panel. Results shown are mean ± SD of three independent experiments. Scale bar, 100 μm. Red: DiI-AcLDL staining. *P < 0.05, **P < 0.01.