Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 15;11(6):462. doi: 10.1038/s41419-020-2668-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a, b The dynamic expression levels of smad7 and TGIF2 at the mRNA and protein levels were quantified by real-time PCR and western blot, respectively. hiPSCs were infected with miR-181c-5p or negative control lentivirus and then induced into IPCs. The data are expressed as the mean ± SD of three independent experiments (n = 7). ^*p ≤ 0.05, ^**p ≤ 0.001, relative to the hiPSCs control. c Endogenous expression levels of smad7, TGIF2, and phosphorylation-smad2/3 were analyzed by western blot. d The effect of phosphorylation-smad2 and phosphorylation-smad3 inhibitors on differentiated hiPSCs was evaluated by flow cytometry. Inhibitor: S1, inhibitors only used in stage 1; Inhibitor: S2-S4, inhibitors used from stage 2 to stage 4; Inhibitor: S5, inhibitors only used in stage 5. LY2109761, phosphorylation-smad2 inhibitor. SIS3 HCl, phosphorylation-smad3 inhibitor. e Proposed mechanism for the regulation of hiPSCs differentiation into IPCs by microRNA-181c-5p.