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. 2020 Jun 15;11:3063. doi: 10.1038/s41467-020-16677-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — The interaction between the droplet boundary and actomyosin networks in the bulk space was modulated by changing the surface property. a Schematic illustration showing alternations of the surface property of droplets. (Top) PEG30-DPHS, a polyethylene glycol (PEG)-based surfactant, will decrease physical interactions of bulk actomyosin networks with the droplet boundary. (Bottom) The VCA domain of WASP conjugated with a histidine-tag was anchored to the droplet boundary via Ni-NTA-conjugated lipids. VCA recruits Arp2/3 and activates its actin nucleation activity. Thus, it was expected that interactions of bulk actomyosin networks with the droplet boundary increased. b The edge-positioned probabilities were compared between different surface properties. Cyan circles show the control data (egg PC). (Top) Passivated droplet surface promoted the cluster centering, whereas (bottom) activated droplet surface promoted the edge positioning. Kolmogorov–Smirnov test was applied to the scatter plots of DC-ratio between D_droplet = 50 μm – 150 µm (around the transition point in the control experiment). The distributions were significantly different from the control in both conditions (p < 0.001). We performed two independent experiments.