Table 1.
Products added to the canine primary sublingual epithelial cells.
| Group of products | Name (specific receptor) | Working concentration | Supplier |
|---|---|---|---|
| Allergen extract | Dermatophagoides farina (XPB81D3A2.5, lot: 307244) | 20 μg dry protein weight (bradford assay)/mL (2.1 μg/mL Der f 1) |
Greer laboratories, Lenoir, NC, USA |
|
Dermatophagoides pteronyssinus (XPB82D3A2.5, lot: 346230) |
20 μg dry protein weight (bradford assay)/mL (0.58 μg/mL Der p 1) |
||
| TLR ligands | FSL-1 (TLR2) | 1 μg/mL | Invivogen, San Diego, CA, USA |
| Heat-killed Listeria monocytogenes (HKLM) (TLR2) | 108 cells/mL | ||
| Pam3CSK4 (TLR2) | 1 μg/mL | ||
| Polyinosinic:polycytidylic acid high molecular weight (poly I:C HMW) (TLR3) | 10 μg/mL | ||
| Polyinosinic:polycytidylic acid low molecular weight (poly I:C LMW) (TLR3) | 10 μg/mL | ||
| LPS (Escherichia coli K12) (TLR4) | 10 μg/mL | ||
| Vitamin D | Calcitriol (vitamin D receptor) | 1–0.01 μM | Merck, Burlington, MA, USA |