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. 2020 Jun 9;8:321. doi: 10.3389/fcell.2020.00321

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Copyright © 2020 López, Marinaro, de Pedro, Sánchez-Margallo, Gómez-Serrano, Ponath, Pogge von Strandmann, Jorge, Vázquez, Fernández-Pereira, Crisóstomo, Álvarez and Casado.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro peripheral blood lymphocyte (PBL) activation and differentiation assays in co-culture with EV-CDCs and IFNγ/EV-CDCs. Peripheral blood lymphocytes (PBLs) were isolated from blood samples by density gradient and co-cultured with 0 (control), 50, 100, and 200 μg/ml of EV-CDC (gray bars) and IFNγ/EV-CDC (black bars) protein for 3 days. Lymphocyte activation/differentiation was analyzed by flow cytometry on CD4+ (A,C) and CD8+ T-cell subpopulations (B,D), using naïve T-cell markers (CD45RA+, CD27+) and activation marker (SLAII). Paired t-test was used to compare doses of EVs to negative controls (*p < 0.05).