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. 2020 Jun 15;10:9608. doi: 10.1038/s41598-020-66286-1

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© The Author(s) 2020, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SDS-PAGE of the purification steps of Neoditomiya sp SBF: (A) crude extract; (B) outflow from anion exchange chromatography; (C) wash; (D) 100 mM NaCl; (E) 200 mM NaCl; (F) 300 mM NaCl and (MW) molecular weight markers. SBF activity predominates between the 100 and 200 mM NaCl eluted fractions.