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. 2020 Jun 15;10:9608. doi: 10.1038/s41598-020-66286-1

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© The Author(s) 2020, corrected publication 2023

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(Left) SDS-PAGE purification steps of the Orfelia luciferase: (A) 0–40% ammonium sulphate precipitated fraction; (B) 40–75% ammonium sulphate luciferase-enriched fraction; (C) 200–300 mM NaCl anion-exchange chromatography eluted luciferase and (D) luciferase isolated from Native-PAGE (right panel), concentrated and re-electrophoresed on denaturing SDS-PAGE; (Right): Photographic image of the Native-Page of Orfelia partially purified luciferase: (A) lane of purified Orfelia luciferase. The luminescence of the luciferase band was revealed after spreading Orfelia hot extract over the gel following by photography with a Canon Ei5 camera upon exposure during 20 s at high sensitivity (3200 ISO).