Table 3.
Growth of kidney derived cultures of IC:20:001 and HB15B203 in semi-solid media at 37 °C.
| Strain | Medium | PID 3 | PID 7 | PIW 2 | PIW 3 | PIW 4 |
|---|---|---|---|---|---|---|
| IC:20:001 | EMJH | — | — | — | — | — |
| IC:20:001 | T80/40/LH | — | — | — | + (6/12) | + (6/12 |
| IC:20:001 | HAN | — | D | D | D | D |
| HB15B203 | EMJH | — | — | — | — | — |
| HB15B203 | T80/40/LH | — | — | — | — | — |
| HB15B203 | HAN | — | + (3/12) | D | D | D |
L. interrogans strain IC:20:001 and L. borgpetersenii strain HB15B203 were inoculated into EMJH, T80/40/LH or HAN semi-solid media incubated at 37 °C. Cultures were visually assessed for growth at post-inoculation day (PID) 3 and 7, and post-inoculation week (PIW) 2, 3 and 4. Cultures were scored as having no visible growth (-), visible growth confirmed by dark-field microscopy (+) or a visible Dinger zone (D) confirmed by dark-field microscopy. Results are derived from three replicates of kidney culture from four hamsters (N = 12) unless indicated in parentheses.