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. 2020 Jun 15;9(6):60. doi: 10.1038/s41389-020-00244-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a U2OS cells treated with tubercidin or mock treated (dimethyl sulfoxide: DMSO) were subjected to immunofluorescence staining with the anti-SC35 antibody. Scale bar: 10 μm. b U2OS cells were incubated with tubercidin or DMSO followed by treatment with CPT. Cell extracts were subjected to immunoblotting analysis with the indicated antibodies. c Screen for nuclear speckle factors involved in HR regulation. The DR-GFP assay was performed with siRNA pools targeting nuclear speckle factors. The siRNA targeting CtIP or luciferase (control: Ctrl) was used as a positive or negative control, respectively, and indicated with blue markers. Homology directed repair efficiency is plotted as a Z-score. The inset magnifies the data for top hits with gene symbols. The genes previously reported to be involved in HR regulation are indicated with green. d A DR-GFP assay was carried out with two independent siRNAs targeting selected top hits from the initial screen. GFP-positive cell populations were normalized to mock treatment (Ctrl), which was set to 100% (mean ± SEM, n = 3). *p < 0.05. **p < 0.01. ***p < 0.005.