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. 2020 Jun 15;9(6):60. doi: 10.1038/s41389-020-00244-4

Fig. 3. USP42 is required for efficient recruitment of BRCA1 to DSB sites.

Fig. 3

a BRCA1 foci formation efficiency was examined with cells transfected with the indicated siRNAs. (Left) The representative images are shown. (Right) The population of cells with >10 BRCA1 foci was plotted (mean ± SEM, n = 3). The cells positive for CENPF staining were analysed. See Fig. S3 for the representative images of undamaged condition. b 53BP1 foci formation efficiency was examined with cells transfected with the indicated siRNAs. The number of foci per nucleus after IR were plotted as a Box and Whiskers plot (median, 5–95 percentile, n = 3). The cells positive for CENPF staining were analysed. c U2OS or USP42 KO cells were transfected with plasmids encoding either GFP or GFP-MRE11. Cell extracts were subjected to immunoprecipitation with an anti-GFP antibody followed by immunoblotting analysis with the indicated antibodies. ***p < 0.005.