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. 2020 Jun 15;10:9652. doi: 10.1038/s41598-020-66670-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NbrgsCaMps have various levels of activity. (a) GUS histochemistry analysis revealed various promoter activities of NbrgsCaMs in seedlings of the NbrgsCaMp::GUS transgenic N. benthamiana. (b) RT-qPCR results of the GUS expression level confirmed the histochemistry results. The relative GUS expression level of NbrgsCaMp7::GUS transgenic N. benthamiana plants was set as 1, as it showed no detectable GUS staining. The relative GUS expression levels of the other NbrgsCaMp::GUS transgenic N. benthamiana plants were calculated using that of the NbrgsCaMp7::GUS transgenic N. benthamiana plants as control. (c) The promoter activity of NbrgsCaM4 is tissue-specific. The GUS staining accumulates mostly in veins and roots. Bars = 5 mm.