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. 2020 Jun 15;10:9652. doi: 10.1038/s41598-020-66670-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Elevated activity of NbrgsCaMp4 during aging and under damaging treatment. (a) The βC1 transgenic plants have deformed needle-like leaves. It was also severely dwarfish compared to the WT. (b) The βC1 transgenic plants had higher NbrgsCaM4 expression than WT. (c) The NbrgsCaMp4::GUS transgenic N. benthamiana plants at the age of 30 and 60 days post germination. (d) The relative GUS expression levels of the plants shown in c. (e) Leaves 1, 2 and 3 (L1, 2 and 3) are selected for the analysis of promoter activity in the WT and NbrgsCaMp4::GUS transgenic N. benthamiana plants. (f) The relative expression levels of NbrgsCaMp4 in L1, 2 and 3 of the WT plants. (g) The relative expression levels of GUS in L1, 2 and 3 of the NbrgsCaMp4::GUS transgenic N. benthamiana plants. (h) Seedlings of the WT and βC1 transgenic plants. (i) The relative expression levels of NbrgsCaMp4 in seedlings of the WT and βC1 transgenic plants. (j) Leaves of the NbrgsCaMp4::GUS transgenic N. benthamiana plants infiltrated with inoculation buffer. Left column, a leaf with infiltration wound and a leaf without treatment that served as the control. Right column, DAB staining of the infiltrated and control leaves. (k) GUS staining of the NbrgsCaMp4::GUS transgenic N. benthamiana leaves at 1 h and 3 h after infiltration. (l) The relative expression levels of GUS in infiltrated NbrgsCaMp4::GUS transgenic N. benthamiana leaves.