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. 2020 Jun 15;11:3034. doi: 10.1038/s41467-020-16669-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Side-view of the RcGTA baseplate. Domains of one hub and one megatron protein are colored according to the sequence diagrams shown on the left and at the bottom of the panel. The iron–sulfur cluster in the hub protein is shown as dark red spheres. Electron densities of tail fibers are shown. The density of one of the fibers is colored according to domains as indicated in the sequence diagram on the right of the panel. b Detail of iron–sulfur cluster coordinated by four cysteines of hub protein. The electron density map of the cluster is stronger than that corresponding to the surrounding proteins. Distances between sulfur atoms of cysteine sidechains (yellow) and iron ions (red) are indicated. c Cryo-EM map of the RcGTA baseplate viewed along its axis towards the head is rainbow-colored based on the distance from the threefold axis of the structure. The inset shows detail of the constriction of the central channel formed by iris/penetration domains of megatron proteins. d The iris/penetration domains from three subunits of megatron proteins are differentiated by shades of red. Interatomic distances between sidechains of Phe17 are indicated. e Domain swapping among baseplate proteins of RcGTA and phage T4. Attachment and oligosaccharide-binding domains of the RcGTA hub protein and the central domain of the megatron protein of RcGTA can be superimposed onto the central hub protein of bacteriophage T4 shown in gray (PDB 1K28).