Skip to main content
. 2020 Jun 15;3:308. doi: 10.1038/s42003-020-1025-y

Fig. 5. Production of in vitro-expanded ASGs-derived offspring using triploid recipients.

Fig. 5

a F1 offspring hatchlings produced by a cross-breeding experiment with wild-type (WT) diploid trout, triploid recipients that received in vitro-expanded ASGs (Cul-TP), and triploid recipients that received freshly prepared testicular cells (Non-cul-TP). b Fluorescence micrographs of the genital ridges of F1 offspring produced from triploid male or female recipients that received in vitro-expanded ASGs. As these ASGs were derived from dominant orange-colored (heterozygous, Orange/WT) vasa-gfp transgenic trout (heterozygous, GFP/−), F1 offspring exhibited four phenotypic patterns: orange body color with GFP-negative germ cells (Orange/Gfp−), orange body color with GFP-positive germ cells (Orange/Gfp+), black-pigmented body color with GFP-negative germ cells (Black/Gfp−), or black-pigmented body color with GFP-positive germ cells (Black/Gfp+). Higher magnification views of the red insets are also shown with red frames. c DNA contents of a wild-type diploid trout (2N cont), F1 juvenile (F1), and triploid trout (3N cont). d Representative picture of transparent skeleton specimens of an F1 offspring from triploid male/female recipients (F1) and a wild-type diploid trout (2N cont). e The number of vertebrae and dorsal, caudal, and anal fin rays in F1 offspring from triploid male/female recipients (F1) and wild-type diploid trout (2N cont). f Karyotype of an F1 offspring (F1; 2n = 60) and wild-type diploid trout (2N cont; 2n = 60). The F1 offspring possessed the same karyotype as that of donor trout (2n = 60). g Seven-month-old F1 offspring developed from expanded ASG-derived sperm and eggs.