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. 2020 Jun 9;11:1134. doi: 10.3389/fmicb.2020.01134

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Copyright © 2020 Zhang, Fu, Han, Xu, Weng, Yan, Liu, Hua, Chen and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Log₂ fold changes of (A) bla_OXA–₄₉₉ and (B) bla_OXA–₈₂₆ expression levels in mutants undergoing or not imipenem selection pressure. RT-qPCR analysis was performed with three biological and technical replicates per experiment. The 2^–ΔΔCt method was applied to calculate the fold change using rpoB as the reference gene and Acinetobacter pittii A1254 as the reference strain. The expression level was calculated as the log₂ fold change. The bars represent means ± SD from triplicate biological repeats. The P-values (each mutant undergoing or not imipenem selection pressure) were determined by two-tailed Student’s t-tests. **P < 0.008; ns, not significant. IPM, imipenem.