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. 2020 Jun 9;11:1067. doi: 10.3389/fmicb.2020.01067

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Copyright © 2020 Jiang, Wu, Jiang, Xie, Bu, Liu, Zhang and Luo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental results of cellular immunofluorescence. (A) The morphology of cell. Morphology of normal 3D4/21 cells is shown on the right, and that of the cells inoculated with the rescued PCV3 after 72 h is on the left. (B) Cell immunofluorescence of D17 mAb to delve into the PCV3 infection with 3D4/21 cells. The data of D17 mAb is represented on the left side and the detection of normal cell is on the right. (C) Nuclear staining results of 3D4/21 cells. The left side represents the nuclear staining pictures in the left of panel (B), and the right side is the relevant one in the right. (D) Results of image superposition. The cell pictures in the corresponding position of panels (B,C) are superimposed. The cell pictures of panel (D) comply with the cell positions of panels (B,C).