TABLE 2.

Ghost boutons and satellite boutons analysis in confocal microscopy.

Genotype	WT	dnrx	dnlgl	dnlg2	dnlg3	dnlg4	OE-dnrx	OE-dnlgl	OE-dnlg2	OE-dnlg3	OE-dnlg4	dnlg2;dnlg3
Ghost boutons per 6/7muscles	2/32	35/16	6/8	10/16	8/8	16/16	12/12	5/8	10/24	5/8	16/24	21/12
Ghost boutons (SEM)	0.06 ± 0.04	2.19 ± 0.43	0.75 ± 0.25	0.63 ± 0.15	1 ± 0.33	1 ± 0.22	1 ± 0.28	0.63 ± 0.18	0.42 ± 0.10	0.63 ± 0.26	0.67 ± 0.16	1.75 ± 0.39
Ghost boutons P-value		***	***	***	***	***	***	***	**	***	***	***
Satellite boutons per 6/7muscles	0/32	0/16	0/8	0/16	0/8	0/16	16/16	19/12	5/8	9/8	9/8	44/12

^aAll fly strain and analyses are described in section Materials and Methods. ^bGhost boutons and Satellite boutons are the total on the muscle 6 and the muscle 7 in segment A2 and A3. ^cP-values for ghost boutons counts were determined by comparison to third instar wild-type (WT). ^dUsing a standard t-test analysis. SEM, standard error of the mean. ** 0.05 < p < 0.01; *** p < 0.001.