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(A) Experimental setup to assess the effect of conditioned media on U87-MG metabolic activity (B) and cell number (C). Metabolic activity (N = 9 hydrogels) and cell number (N = 12 hydrogels at Day 3, N = 9 hydrogels at Day 7) were expressed at each time point as a fold change compared to Day 0. Fold change values were then normalized to the EBM2 group at Day 3. *P < 0.01, **P < 0.001 between groups.
