TABLE 1.
Deduced functions of the gene products residing in the persiamycin cluster.
| Gene product | RAST annotation | Size (AA) | Most similar ORF [species] (identity %) | Accession no. |
| PerU1 | 3-oxoacyl-[acyl-carrier protein] reductase | 255 | MULTISPECIES: SDR family oxidoreductase [Streptomyces](90) | WP_043485899 |
| PerR1 | LysR-type transcriptional regulator | 297 | LysR family transcriptional regulator [Nocardiopsis sp. JB363] (84) | WP_087099906.1 |
| PerU2 | hypothetical protein | 149 | hypothetical protein [Nocardiopsis sinuspersici] (77) | WP_077691354.1 |
| PerU3 | hypothetical protein | 62 | 2-isopropylmalate synthase [Clostridium asparagiforme] (39) | WP_040410861.1 |
| PerU4 | glucoamylase | 608 | glycoside hydrolase family 15 protein [Amycolatopsis taiwanensis] (80) | WP_027940885.1 |
| PerT1 | major facilitator superfamily MFS_1 | 526 | MFS transporter [Nocardiopsis halophila] (69) | WP_051063540.1 |
| PerR2 | signal transduction response regulator | 1074 | AAA family ATPase [Nocardiopsis halophila] (58) | WP_017537044.1 |
| PerG | O-methyltransferase | 342 | class I SAM-dependent methyltransferase [Nonomuraea sp. KC333] (71) | WP_111179428.1 |
| PerP | NDP-hexose 3-C-methyltransferase TylCIII | 412 | class I SAM-dependent methyltransferase [Nonomuraea sp. KC333] (76) | WP_111179448.1 |
| PerQ | dTDP-4-dehydrorhamnose 3,5-epimerase | 217 | dTDP-4-keto-6-deoxy-D-glucose epimerase [Nonomuraea sp. KC333] (72) | WP_111179449.1 |
| PerM | dTDP-glucose 4,6-dehydratase | 327 | dTDP-glucose 4,6-dehydratase [Nonomuraea sp. KC333] (74) | WP_111179429.1 |
| PerU5 | hypothetical protein | 147 | hypothetical protein [Nonomuraea sp. KC333] (60) | WP_111179451.1 |
| PerN | hypothetical protein | 467 | NDP-hexose 2,3-dehydratase [Nonomuraea sp. KC333] (67) | WP_111179427.1 |
| PerO | aminotransferase | 373 | DegT/DnrJ/EryC1/StrS family aminotransferase [Nonomuraea sp. KC333] (78) | WP_111179426.1 |
| PerK | glycosyltransferase | 404 | DUF1205 domain-containing protein [Nonomuraea sp. KC333] (69) | WP_111179425.1 |
| PerL | glucose-1-phosphate thymidynyltransferase | 300 | glucose-1-phosphate thymidylyltransferase [Nonomuraea sp. KC333] (80) | WP_111179424.1 |
| Per2 | polyketide chain length factor WhiE-CLF paralog | 408 | ketosynthase chain-length factor [Nonomuraea sp. KC333] (74) | WP_111179437.1 |
| Per1 | polyketide beta-ketoacyl synthase WhiE-KS paralog | 425 | beta-ketoacyl-[acyl-carrier-protein] synthase family protein [Nonomuraea sp. KC333] (82) | WP_111179436.1 |
| PerU6 | hypothetical protein | 151 | DUF1772 domain-containing protein [Nonomuraea sp. KC333] (66) | WP_111179435.1 |
| PerC | polyketide cyclase WhiE VII | 107 | TcmI family type II polyketide cyclase [Nonomuraea sp. KC333] (75) | WP_111179434.1 |
| PerI | biotin carboxylase | 465 | acetyl-CoA carboxylase biotin carboxylase subunit [Catenulispora acidiphila] (64) | WP_012785313.1 |
| PerJ | biotin carboxyl carrier protein of acetyl-CoA carboxylase | 164 | acetyl-CoA carboxylase biotin carboxyl carrier protein [Streptomyces thermoautotrophicus] (49) | WP_066890277.1 |
| PerB | aromatase WhiE VI | 162 | polyketide cyclase [Nonomuraea sp. KC333] (72) | WP_111179433.1 |
| PerD | polyketide cyclase WhiE II | 142 | cupin domain-containing protein [Nonomuraea sp. KC333] (70) | WP_111179432.1 |
| Per3 | acyl carrier protein | 85 | acyl carrier protein [Nonomuraea sp. KC333] (67) | WP_111179418.1 |
| PerH | acetyl-CoA carboxyl transferase | 507 | acetyl-CoA carboxylase carboxyl transferase subunit alpha [Nonomuraea sp. KC333] (72) | WP_111179419.1 |
| PerR3 | transcriptional regulator MarR | 154 | DNA-binding transcriptional regulator, MarR family [Thermostaphylospora chromogena] (72) | SDQ92350.1 |
| PerR4 | regulatory protein | 218 | AfsR/SARP family transcriptional regulator [Nonomuraea sp. KC333] (68) | WP_111179423.1 |
| PerA | 3-oxoacyl-ACP reductase | 261 | SDR family oxidoreductase [Nonomuraea sp. KC333] (78) | WP_111179422.1 |
| PerF | FAD binding protein | 415 | NAD(P)/FAD-dependent oxidoreductase [Nonomuraea sp. KC333] (75) | WP_111179417.1 |
| PerU7 | oxidoreductase | 180 | NAD-dependent epimerase/dehydratase family protein [Nonomuraea sp. KC333] (71) | WP_111179431.1 |
| PerU8 | hypothetical protein | 93 | antitoxin [Nocardiopsis baichengensis] (68) | WP_017558586.1 |
| PerE | carboxylesterase NP | 339 | alpha/beta hydrolase [Tsukamurella sp. 1534] (76) | WP_081586088.1 |
| PerR5 | transcriptional regulator TetR-family | 199 | TetR/AcrR family transcriptional regulator [Brevibacterium aurantiacum] (63) | WP_009884360.1 |
| PerU9 | bifunctional deaminase-reductase domain protein | 184 | deaminase [Streptomyces sp. KM273126] (83) | WP_131570927.1 |
| PerU10 | hypothetical protein | 132 | hypothetical protein [Streptomonospora alba] (84) | WP_040271289.1 |
| PerU11 | pterin-4-alpha-carbinolamine dehydratase | 75 | 4a-hydroxytetrahydrobiopterin dehydratase [Haloactinopolyspora alba](80) | WP_106538811.1 |
| PerR6 | putative two-component system sensor kinase | 413 | HAMP domain-containing protein [Streptomonospora alba] (93) | WP_040274336.1 |
| PerR7 | putative two-component system response regulator | 229 | response regulator transcription factor [Streptomonospora alba] (96) | WP_040274337.1 |