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. 2020 Jun 9;11:1225. doi: 10.3389/fmicb.2020.01225

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Copyright © 2020 Yan and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time course of S. putrefaciens WS13 biofilm production for 8, 12, 24, 36, 48, 60, 72, 84, 96, 108, 120, and 132 h at 4, 15, and 30°C. The images were representative of three independent replicates. (A) Representative CLSM images of biofilm formed by S. putrefaciens WS13 after 8, 12, 24, 36, 48, 60, 72, 84, 96, 108, 120, and 132 h incubation. (B) Representative CLSM images of the mature biofilm formed by S. putrefaciens WS13 at 4, 15, and 30°C. (C) The biovolume of the mature biofilm at different temperature. (D) The mean thickness of the mature biofilm at different temperature. The error bar represents the standard deviation of triplicate experiments.