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. 2020 Jun 9;7:287. doi: 10.3389/fvets.2020.00287

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Copyright © 2020 Atchou, Ongus, Machuka, Juma, Tiambo, Djikeng, Silva and Pelle.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Enzyme-linked immunosorbent assay (ELISA) evaluation of the antigenicity of (A) TP04_0076 gene product (recombinant protein TP04_0076F) and predicted antibody target epitopes of TP04_0640 and TP04_0076 gene products (synthetic peptides (B) TP04_0076ep2, (C) TP04_0640ep1, and (D) TP04_0640ep2). A panel of 34 predetermined bovine positive controls with strong percent positivity (PP ≥ 80) to PIM antigen (red line) were included in the analysis. The PP results of each of the four recombinant proteins and synthetic peptides tested (blue line) are shown in separated panels. The green line represents the cutoff, and an assay with a PP ≥ 20 (PP ≥ 20) is considered positive.