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Uptake of lipid analogs Rhodamine-liss phosphatidylethanolamine (Rhodamine-liss PE) (A,B) or fluorescent phosphatidylcholine (NBD-PC) (C,D) normalized by Hoechst 33342 nucleus staining, in FcgRIIB–/– and wild-type (FcgRIIB+/+) macrophages in control (N/N) and LPS-tolerance (100/100) with representative immunofluorescence images (E) are demonstrated (images of NBD-PC uptake are not shown). (Independent triplicate experiments were performed).
