FIG 3.

Transcription factor AP-2β and DHX33 bound to the promoters of a subset of genes that partially overlapped. (A) H1299 cells were subjected to ChIP-seq analysis with anti-AP-2β antibody; IgG was used as a control. Over 3,000 genes were found to associate with AP-2β. The heat map shows the binding region (red) of AP-2β on different gene domains. The scale bar is shown on the right. (B) Data representing the population were compared to ChIP-seq data from anti-DHX33. Gene Venn values (top) and peak Venn values (bottom) are shown. Over 2,800 genes were found to be bound by both DHX33 and AP-2β proteins. (C) KEGG pathway analysis of genes associated with AP-2β protein. (D) RNA sequencing was performed for RNA samples extracted from H1299 cells after AP-2β was knocked down. The FPKM values determined for a subset of genes involved in cell cycle or apoptosis are shown. (E) Western blotting was performed to confirm that AP-2β was efficiently knocked down in H1299 cells. GAPDH served as an internal control.