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. 2020 Apr 29;20(1):367–374. doi: 10.3892/etm.2020.8701

Figure 4.

Figure 4

BA increases the expression levels of SIRT1 and p-p65 in HG-induced podocytes. Following serum starvation for 12 h, podocytes were cultured with HG (30 mM D-glucose) or normal glucose (5 mM D-glucose) for 48 h and then treated with 6.25, 12.5 or 25 µM BA for 24 h. (A) Western blotting was used to analyze the expression levels of SIRT1 at the protein level. (B) mRNA expression levels of SIRT1 in podocytes were determined using RT-qPCR. (C) Western blotting was used to analyze the protein expression levels of p-p65 and p65. (D) Semi-quantification of the ratio between p-p65/p65. (E) mRNA expression levels of p65 in podocytes were analyzed using RT-qPCR. All data are presented as the mean ± SD. **P<0.01 vs. NG group. #P<0.05 and ##P<0.01 vs. HG treatment group. BA, baicalin; HG, high glucose; p, phosphorylated; RT-qPCR, reverse transcription-quantitative PCR; SIRT1, sirtuin 1; NG, normal glucose.