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. 2020 Jun 9;31(10):107697. doi: 10.1016/j.celrep.2020.107697
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
NCX1 SWANT Cat#R3F1; RRID:AB_2716744
FLOT2 BD Biosciences Cat#610384; RRID:AB_397767
DHHC5 Sigma Cat#HPA014670; RRID:AB_2257442
GFP Abcam Cat#ab32146; RRID:AB_732717
Chemicals, Peptides, and Recombinant Proteins
NCX1 peptide (NCX1740-756) with N-terminal biotin Alta Biosciences N/A
DHHC5 peptides (C-tail: DHHC5213-237,223-247,233-257,243-267,253-277,263-287,273-297,283-307,293-317,303-327,313-337,323-347,333-357) all with N-terminal biotin Alta Biosciences N/A
XIP (WT and K229Q XIP; NCX1219-238) with N-terminal biotin Alta Biosciences N/A
PalmB Calbiochem Cat#178501
ML348 Dr. Brent Martin, University of Michigan N/A
ML349 Dr. Brent Martin, University of Michigan N/A
Filipin Sigma Cat#F9765
Ctx Alexa Fluor 647 Invitrogen Cat#C34778
FAST-Dil Invitrogen Cat#D7756
Palmitic Acid Sigma Cat#0500
2-Bromopalmitate Sigma Cat#238422
Methyl-β-cyclodextrin Sigma Cat#332615
Critical Commercial Assays
Fluo-4 Direct Calcium Imaging Invitrogen Cat#F10472
Experimental Models: Cell Lines
FT-293 Invitrogen Cat#R70007
HEK293T ATCC CRL-1573
Experimental Models: Organisms/Strains
New Zealand White Rabbit Envigo 444
Sprague Dawley Rat Envigo 002
Oligonucleotides
NCX1 Δ709-728 cloning, forward: 5′-GGACAAACGATGAA
TGTGGAGAGGAGAAGC-3′		 Eurofins Genomics N/A
NCX1 Δ709-728 cloning, reverse: 5′-ATTCATCGTTTGTCC
CAACCACAAGGGC-3′		 Eurofins Genomics N/A
C739A NCX1 mutagenesis, forward: 5′-TGCATCAC
ATAATCGAAAGCGGAGGGCAGCTTCTCCTC-3′		 Eurofins Genomics N/A
C739A NCX1 mutagenesis, reverse: 5′-GAGGAGAAG
CTGCCCTCCGCTTTCGATTATGTGATGCA-3		 Eurofins Genomics N/A
Recombinant DNA
Canine NCX1.1 cDNA Kenneth Philipson, UCLA N/A
Canine NCX1.1 with CFP or YFP inserted at amino acid 266 Michela Ottolia, UCLA N/A
YFP fusion proteins to the N terminus the NCX1.1 intracellular loop Reilly et al., 2015, Plain et al., 2017 N/A
Software and Algorithms
GraphPad Graphpad Prism v6 https://graphpad.com
ImageJ / Fiji Schindelin et al., 2012 https://imagej.nih.gov/ij/
The Discovery Series Quantity One 1-D Analysis Software Version 4.6.6, PC Bio-Rad LIT-70-9600-Q1-466PC