Figure 3.
Distinct LHb Neuronal Ensembles during Defensive Behaviors
(A) Top: schematic of the experiment. Bottom, pictures showing mouse with miniscope attached, GRIN lens placement, GCaMP6f expression, field of view with identified cells (maximum intensity projections), map of active LHb neurons, and respective sample traces (right).
(B) Mean Ca2+ responses (Z score) across runaway (left) and action-locking (right) trials for 46 LHb neurons imaged within a single mouse, aligned to the onset of the behavioral reaction. Highlighted at top, the average response of a single cell (Cell: 15). Bottom, averaged time course of all cells for runaway and action-locking strategies.
(C) Cluster identification by unsupervised classification during runaway (top) and action-locking (center), including all neurons recorded. Bottom, average trace across all of the neurons within the cluster. Plots are aligned to the action onset.
See also Figure S4.