Figure 4.

Increased Synaptic LiGluK2 Receptor Mobility Affects Glutamatergic Synaptic Currents

(A) Top: stimulating protocol: train of 6 brief (0.8 ms) consecutive UV light pulses at 20 Hz to uncage MNI-glutamate. Bottom: representative traces of LiGluK2-mediated uEPSCs in control (left) and X-link conditions (right).

(B) Representative traces of uEPSCs mediated by LiGluK2Δ16 in control (left) and X-link conditions (right).

(C) Representative traces of uEPSCs evoked in N-cadΔE/LiGluK2-expressing neurons in control (left) and X-link conditions (right).

(D) Left: the percentage of desensitization of uEPSCs is larger in LiGluK2- (black) expressing neurons than in LiGluK2Δ16- (gray) and N-cadΔE/LiGluK2- (cyan) expressing neurons. Right: percentage of desensitization of synaptic uEPSCs as on the left, upon receptor X-link. Receptor immobilization by X-link did not change the control (LiGluK2) currents but reverted the desensitization of currents evoked in LiGluK2Δ16- and N-cadΔE/LiGluK2-expressing neurons to control values.

Data are presented as mean ± SEM, ^∗p < 0.05; ns, non-significant.