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. 2020 Feb 19;3(3):535–546. doi: 10.1021/acsptsci.0c00002

Figure 2.

Figure 2

Activity of Pn3a mutants at hNaV1.7 assessed by whole-cell patch–clamp experiments. (A) Potency of Pn3a (WT) and the surface hydrophobic patch analogues Y4A, Y27A, and W30A. Y27A is in a non-native fold. (B) Potency of analogues with mutations at position 22 and 24. (C) Potency of charged ring analogues D8G, D8K, D8N, and D1K. (D) Potency of Pn3a analogues E10K, E13K, E13A, D12T, and D14K. D12T and D14K are in a non-native fold. The dotted line indicates the maximal tested concentration of 1 μM. The dashed line indicates the potency of wild-type Pn3a. Data are presented as mean ± SEM, with n = 3–9 cells per data point.