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. 2020 Jun 11;14:2319–2334. doi: 10.2147/DDDT.S250061

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© 2020 Dokduang et al.

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Varlitinib induces CCA cell apoptosis.

Notes: (A and B) KKU-214 and KKU-100 cells were treated with varlitinib at the indicated concentrations after which the apoptotic cells were counted using the Annexin-V-FLUOS staining kit and flow cytometry. Live cells stained annexin V−/PI− (left bottom panel) and apoptotic cells stained annexin V+/PI− or annexin V+/PI+ (right bottom and right top panel). (C) A caspase-3 activity assay was performed on KKU-214 and KKU-100 cells after treatment with the designated concentrations for 72 hrs. Data in (B and C) are represented as mean ± SD of three independent experiments. Asterisks indicate statistical significance (p <0.001) compared to the control, analyzed by one-way ANOVA.