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. Author manuscript; available in PMC: 2020 Oct 22.
Published in final edited form as: Nature. 2020 Apr 22;581(7806):100–105. doi: 10.1038/s41586-020-2229-5

Extended Data Figure 3 |. Inhibition of macroautophagy, but not LAP/LANDO, restores MHC-I levels.

Extended Data Figure 3 |

Knockdown of FIP200, ATG14, Atg13, and Ulk1, but not Rubicon, increased MHC-I levels in PDAC cells. a,d,g,i,k, Knockdown efficiency was confirmed by immunoblot (a,i,k) and qPCR (d,g). Data are mean ± s.d. from three biological replicates per group (d,g). a,e, Whole cell abundance of MHC-I was assessed by immunoblot. c, Immunofluorescence staining of MHC-I (green) and LAMP2 (red). Scale bar, 50 μm. b,f,h,j,l, Cell surface MHC-I levels were measured by flow cytometry (b,f, n = 9; h,j, n = 12; l, n = 16). Data are pooled from at least three independent experiments. Graphs are mean ± s.d. a-f, PaTu8902 cells (human). g-l, HY15549 cells (mouse). A representative of at least two independent experiments is shown in a,c,e,i,k. P values determined by unpaired two-tailed t-tests. For gel source data of a,e,i,k, see Supplementary Fig. 1.