Table 1.
Mutation frequencies induced by SWISSv3 in T0 rice plants
| Types of genome editing | Target sites | No. of transgenic rice lines | No. of mutantsa | Genotype of mutationsb | Heterozygous/homozygous |
|---|---|---|---|---|---|
| CBE | OsALS-T2 | 55 | 14 (25.5%) | C6>T6 (7); C6>G6 (2); C6C7>T6T7 (4); C6C7C16>T6T7T16 (1) | 14/0 |
| ABE | OsACC-T2 | 9 (16.4%) | A4>G4 (7); A7>G7 (2) | 9/0 | |
| Indels | OsBADH2-Indels | 29 (52.7%) | deletions (22); insertions (7) | 19/10 |
aBased on the number of T0 lines (rice) carrying the observed mutations over the total number of T0 transgenic rice lines analyzed
bThe genotypes of indels were analyzed with the online tools DSDecodeM and TIDE (see the “Methods” section)