Fig. 2.

FBXO2 regulates the OS cells proliferation in vitro and in vivo. a FBXO2 protein expression in MG63 cells with or without Flag-FBXO2 overexpression. b Cell growth curve of MG63 cells with or without Flag-FBXO2 overexpression. c Colony formation assay of MG63 cells with or without Flag-FBXO2 overexpression. d FBXO2 KO U2OS cells were generated by CRISPR assay and detected by western blot. e Cell growth curve of FBXO2 WT or FBXO2 KO U2OS cells. f Colony formation assay of FBXO2 WT or FBXO2 KO U2OS cells. g 1 × 10⁷ FBXO2 WT or KO U2OS cells were injected subcutaneously into BALB/c nude mice, tumour growth was measured using a caliper at the indicated times after injection. n = 5 for each group. h Mice were sacrificed 6 weeks after transplantation. The tumors were then excised and weighed and subjected to western blot assay with indicated antibodies