Fig. 3.

Phylogenetic relationship, gene structure and conserved motif analyses of BrPERK genes. a, Phylogenetic tree of BrPERK proteins’ sequences was generated by unrooted NJ, and the bootstrap test replicate was set as 1000 times with MEGA7. b, Conserved motifs of BrPERK proteins that were identified using the MEME program. Ten putative motifs in Fig. S3 are indicated by different coloured boxes. c, The exon-intron organization and Pkinase domain (and STKc_IRAK) of BrPERK genes. Black lines represent introns. Exons, Pkc_like superfamily domains and STKc_IRAK domains are represented by purple, orange and yellow boxes, respectively