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. 2020 Jun 15;21:401. doi: 10.1186/s12864-020-06802-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — RNA-Seq and qRT-PCR analyses of the BrPERK genes in B. rapa. a, Illumina RNA-Seq data analysis for six tissues of B. rapa. b, qRT-PCR analysis of BrPERK genes in different tissues from B. rapa, the gene expression values of different PERK genes in leaf were set as 1. The bars on the top represent of RPKM values and relative expression values, respectively. c, Cytological observation of five stages (meiosis II period, tetrad period, uninucleate period, binucleate period and mature pollen period) of anther development in fertile plant of wucai (B. rapa); MSp, microspore; PG, pollen grain; T, tapetum. d, qRT-PCR analysis of several BrPERK genes in the five periods of anther development from wucai fertile plant