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. 2020 Jun 15;21:401. doi: 10.1186/s12864-020-06802-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — RNA-seq and qRT-PCR analyses of BrPERK genes in several male sterility lines of B. rapa. a, Heat map analysis of BrPERK genes in the six different male sterility lines, all the DEGs of BrPERK genes were down-regulated; seven (Bra001723.1, Bra011534.1, Bra016342.1, Bra018431.1, Bra022359.1, Bra024608.1 and Bra039607.1) of 14 BrPERK DEGs were downregulated in all male sterility plants; the color bar on the top represents fold change values.b. Transverse sections of anther development in five periods (meiosis II period, tetrad period, uninucleate period, binucleate period and mature pollen period) from Wucai ogu-CMS plant; MSp, microspore; T, tapetum. c, qRT-PCR analysis of down-regulated BrPERK genes in different anther development phases of B. rapa ogu-CMS plants (Wucai)