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. 2020 Jun 15;20:161. doi: 10.1186/s12866-020-01848-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The effect of 17ß-estradiol on the yeast-sensitivity of Culex quinquefasciatus mosquitoes. a Multiple amino acid sequence alignment between D. melanogaster Estrogen Related Receptor (dmERR) and C. quinquefasciatus ERR (cqERR). The sequences of dmERR (Accession NP_648183) and cqERR (Accession EDS37237) were aligned using MultAlin software as in Fig. 1b. The DNA binding domain and ligand binding domain are highlighted in blue and green boxes, respectively. Identical amino acids are shown in red. The extent (%) of the identity/similarity between Drosophila and Culex sequences is shown above the alignment. b Female C. quinquefasciatus mosquitoes were orally challenged with 3.3 × 10⁷S. cerevisiae cells/ml with and without 75 mM of 17ß-estradiol (17ß-E). P-value indicates statistical significance compared to the yeast-only condition (asterisks) on the basis of the Log-rank (Mantel-Cox) test