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. 2020 Jun 16;18:67. doi: 10.1186/s12915-020-00791-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Loss of ACL/ACS or BCKDH results in predominant hypo-acetylation of nucleo-cytosolic and mitochondrial proteins, respectively. a Volcano plot highlighting differentially acetylated sites of ΔACL/iΔACS compared to RH parasites. Statistically significant differences between parasite lines were determined as outlined in the ‘Material and methods’ section. Unchanged acetylation sites are displayed in black, while sites which are significantly hyper- or hypo-acetylated in ΔACL/iΔACS (≥ 2-fold, n = 3, limma p < 0.01) are displayed in red and blue, respectively. b Hyper- and hypo-acetylated sites in ΔACL/iΔACS parasites were sorted according to their sub-cellular localisation using the hyperplexed Localisation of Organelle Proteins by Isotopic Tagging (hyperLOPIT) data available under https://proteome.shinyapps.io/toxolopittzex/ [27]. c Volcano plot highlighting the differentially acetylated sites in ΔBCKDH compared to RH parasites. Statistically significant differences were determined as outlined in the ‘Material and methods’ section. Unchanged acetylation sites are displayed in black, while sites which are significantly (≥ 2-fold, n = 3, limma p < 0.01) hyper- or hypo-acetylated in ΔBCKDH are displayed in red and blue, respectively. d Hyper- and hypo-acetylated sites in ΔBCKDH parasites were sorted according to their sub-cellular localisation using hyperLOPIT data available under https://proteome.shinyapps.io/toxolopittzex/ [27]. BCKDH, branched-chain α-keto acid dehydrogenase-complex; ACL, ATP citrate lyase; ACS, acetyl-CoA synthetase; PM, plasma membrane; IMC, inner membrane complex; ER, endoplasmic reticulum