Table 1.
Data Collection and Refinement statistics of apo and 5’-AMP bound RNase 6
| Data Collection | RNase 6 | RNase 6/5’-AMP |
|---|---|---|
| Space group | P22121 | P212121 |
| Unit cell (Å) | 27.8, 38.7, 108.5 | 27.6, 38.8, 97.9 |
| Resolution (Å) | 26.5 – 1.5 (1.55 – 1.5) | 36.1 – 2.59 (2.71–2.59) |
| Rmeas | 4.8 (28.3) | 4.6 (8.3) |
| I / σI | 14.5 (8.1) | 17.2 (10.4) |
| Completeness (%) | 99.1 (98.9) | 98.6 (97.4) |
| Redundancy | 3.1 (2.4) | 2.4 (2.0) |
| Refinement | ||
| Resolution (Å) | 26.5 – 1.5 | 36.1 – 2.6 |
| No. reflections | 19372 | 3537 |
| Rwork / Rfree | 17.3/22.8 | 22.0/25.1 |
| Number of Atoms | ||
| Protein | 1868 | 1023 |
| Water | 108 | 27 |
| Phosphate | 15 | - |
| AMP | - | 32.3 |
| B-factors (Å2) | ||
| Protein | 16.8 | 19.0 |
| Water | 30.1 | 17.3 |
| Phosphate | 44.7 | - |
| AMP | - | 23 |
| RMSD | ||
| Bond lengths (Å) | 0.006 | 0.003 |
| Bond angles (°) | 1.048 | 0.703 |
| Molprobity scores | 1.5 | 1.43 |
| Ramachandran favored (%) | 100 | 99.2 |
| Ramachandran allowed (%) | 0 | 0.8 |
| PDB ID | 6MV6 | 6MV7 |
a
Values in () are for the last resolution shell.